Yeast CRISPRi guide selection and evaluation
This site helps to select effective guide RNAs for dCas9-mediated transcriptional repression, and evaluate existing designs for efficacy. The estimates are based on the work of Smith et al. (2016), that derived several yeast-specific guide RNA design rules for CRISPRi, and publically available nucleosome occupancy, ATAC-seq, and transcription start site data.
Citation
If you use the yeast CRISPRi resource in your work, please cite the following:
Smith, J.D., Suresh, S., Schlecht, U., Wu, M., Wagih, O., Peltz, G., Davis, R.W., Steinmetz, L.M., Parts, L. and Onge, R.P.S., 2016. Quantitative CRISPR interference screens in yeast identify chemical-genetic interactions and new rules for guide RNA design. Genome biology, 17(1), p.1.
Link to article
Contact information
For questions or comments, please get in touch with one of us
- Justin Smith
- j...@gmail.com
- Omar Wagih
- w...@ebi.ac.uk
- Leopold Parts
- l...@sanger.ac.uk
- Robet P. St. Onge
- b...@stanford.edu