This site helps to select effective guide RNAs for dCas9-mediated transcriptional repression, and evaluate existing designs for efficacy. The estimates are based on the work of Smith et al. (2016), that derived several yeast-specific guide RNA design rules for CRISPRi, and publically available nucleosome occupancy, ATAC-seq, and transcription start site data.
If you use the yeast CRISPRi resource in your work, please cite the following:
Smith, J.D., Suresh, S., Schlecht, U., Wu, M., Wagih, O., Peltz, G., Davis, R.W., Steinmetz, L.M., Parts, L. and Onge, R.P.S., 2016. Quantitative CRISPR interference screens in yeast identify chemical-genetic interactions and new rules for guide RNA design. Genome biology, 17(1), p.1.
Posterior probability of nucleosome occupancy as defined in Schep et al. (2015) Genome Research
Read count from Schep et al. ATAC-seq experiment, averaged in a sliding window of 50bp, and normalised to the largest value in a 1000bp window. This features encodes how open the chromatin is relative to the rest of the region.